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一步法人催乳素(PRL)酶联免疫吸附检测试剂盒
发布时间：2024-12-06 浏览次数：12次
Overview
| Product name: | EasyStep Human PRL(Prolactin) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | LTH; Luteotropic Hormone |
| Assay Type: | Sandwich |
| Sensitivity: | 248 pg/mL |
| Standard: | 20 ng/mL |
| Detection Range: | 0.32-20 ng/mL |
| Sample Type: | Serum |
| Assay Length: | 1.5h |
| Research Area: | Endocrinology; Reproductive science; Genetic science; Hormone metabolism |
| Uniprot ID: | P01236 |
| Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRL, and the Human PRL standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PRL. After TMB substrate solution is added, only those wells that contain Human PRL and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRL in the samples is then determined by comparing the OD of the samples to the standard curve. |
标准曲线
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 20.00 | 2.121 | 2.036 |
| 10.00 | 1.586 | 1.501 |
| 5.00 | 1.196 | 1.111 |
| 2.50 | 0.835 | 0.750 |
| 1.25 | 0.582 | 0.497 |
| 0.63 | 0.315 | 0.230 |
| 0.32 | 0.129 | 0.044 |
| 0.00 | 0.085 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant PRL and the recovery rates were calculated by comparing the measured value to the expected amount of PRL in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 80-92% | 86% |
| EDTA plasma(n=5) | 85-97% | 91% |
| Heparin plasma(n=5) | 83-95% | 89% |
线性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PRL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 85-92% | 79-96% | 92-101% | 87-98% |
| EDTA plasma(n=5) | 83-97% | 84-99% | 95-102% | 88-103% |
| Heparin plasma(n=5) | 86-94% | 91-105% | 87-99% | 95-100% |